ALTERATION OF BACTERIOLYTIC ENZYME PROFILE OF STAPHYLOCOCCUS-AUREUS DURING GROWTH

Profiles of cell-associated bacteriolytic activities and those in the culture supernatant of Staphylococcus aureus FDA209P at various stages of growth were analyzed using sodium dodecyl sulfate-polyacrylamide g els containing Micrococcus luteus or S. aureus. In the logarithmic gro wth phase, the cell-associated bacteriolytic activities extracted with Triton X-100 contained a number of bacteriolytic proteins, the profil es of which were similar to those we reported elsewhere (Sugai, M., Ak iyama, T., Komatsuzawa, H., Miyake, Y., and Suginaka, H.(1990) J. Bact eriol., 172, 6494-6498). The proteins include P1, P2, P7, P9, PX, P13, P18 and other minor components. At the stationary growth phase, the b acteriolytic band-profile of the Triton X-100 extract changed dramatic ally. P1, P7 and P9 disappeared, and the other minor bands had markedl y decreased band intensities. On the other hand, P2, PX, P13, and P18 retained their band intensities during the stationary growth phase. Th e band intensities of P7, P13, PX, and P18 increased in the supernatan t during the logarithmic growth phase. These results indicated that th e bacteriolytic band-profile changes during growth.