CONTINUOUS PRODUCTION OF TISSUE-PLASMINOGEN ACTIVATOR FROM RECOMBINANT CHO CELLS IN A DEPTH FILTER PERFUSION SYSTEM

Authors
CHOI SK CHANG HN OH DJ
Citation
Sk. Choi et al., CONTINUOUS PRODUCTION OF TISSUE-PLASMINOGEN ACTIVATOR FROM RECOMBINANT CHO CELLS IN A DEPTH FILTER PERFUSION SYSTEM, Biotechnology techniques, 9(8), 1995, pp. 567-572
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
8
Year of publication
1995
Pages
567 - 572
Database
ISI
SICI code
0951-208X(1995)9:8<567:CPOTAF>2.0.ZU;2-X
Abstract
A depth filter perfusion system (DFPS), equipped with a 40-mu m polypr opylene depth filter for cell immobilization, was used for the continu ous production of tissue plasminogen activator (t-PA) from recombinant Chinese hamster ovary cells. Final cell density in the DFPS with oxyg en control was 1.8x10(7) cells/mL of the total working volume and maxi mum t-PA productivity was 2.63 mg/L/day. Dissolved oxygen concentratio n in the filter matrix was successfully controlled by air sparging and stable operation was possible for more than 20 days.