MICELLAR ELECTROKINETIC CHROMATOGRAPHY USING HIGH-MOLECULAR-MASS SURFACTANTS - COMPARISON BETWEEN ANIONIC AND CATIONIC SURFACTANTS AND EFFECTS OF MODIFIERS

Two high-molecular-mass surfactants, butyl acrylate-butyl methacrylate -methacrylic acid copolymer sodium salt (BBMA) and butyl methacrylate- methacryloyloxyethyltrimethylammonium chloride copolymer (BMAC), were utilized as new pseudo-stationary phases in micellar electrokinetic ch romatography (MEKC). As with BBMA, BMAC was successfully employed as a pseudo-stationary phase, but reversal of the electroosmotic flow was observed. The capacity factors were proportional to the concentration of BMAC, and the critical micelle concentration was effectively zero. The effect of the addition of methanol and a non-ionic surfactant were studied in MEKC with BBMA. Chiral separation was studied by cyclodext rin (CD) modified MEKC with BBMA: enantiomers of dansylated-DL-amino a cids were separated with 2% BBMA and 10 mM beta-CD, giving larger sepa ration factors than those obtained with 100 mM sodium dodecyl sulfate and 60 mM beta-CD.