CAPILLARY ZONE ELECTROPHORESIS OF SERUM-PROTEINS - STUDY OF SEPARATION VARIABLES

Electrophoresis of serum proteins is one of the traditional applicatio ns of zone electrophoresis. Whereas electrophoresis in supporting medi a uses usually 5,5'-diethylbarbiturate at pH 8.6 as the buffer, in cap illary zone electrophoresis with on-line UV detection, this electrolyt e is of little use because of its high UV absorbance. For that reason, a number of operational electrolytes differing in composition were te sted for use in capillary electrophoresis of serum proteins. The influ ence of the pK(A) of co-ions and counter ions and the concentration of the operational electrolyte was examined. If 0.1 M methylglucamine-0. 1 M epsilon-aminocaproic acid or 0.1 M methylglucamine, -0.1 M gamma-a minobutyric acid is used as the operational electrolyte, capillary ele ctrophoresis separates serum proteins into more than ten zones.