ENTERAL HUMAN SERUM IMMUNOGLOBULIN TREATMENT OF CRYPTOSPORIDIOSIS IN MICE WITH SEVERE COMBINED IMMUNODEFICIENCY

The anti-cryptosporidial immunoglobulin G antibodies in two commercial ly available human serum immunoglobulin (HSIG) products were quantifie d and characterized, The mean level of Cryptosporidium parvum-specific immunoglobulin G in HSIG was eightfold higher than the antibody level found in the sera of three immunocompetent individuals convalescing f rom cryptosporidiosis, However, HSIG products displayed no reactivity to cryptosporidial antigens in immunoblot analyses, while convalescent -phase sera demonstrated characteristic banding patterns, When HSIG wa s given to newborn severe combined immunodeficiency (scid) mice before and shortly after experimental infection, a decreased intensity of in fection was observed in the intestines of the mice compared with that of control mice, However, there was no difference in mortality or hist opathologic findings in the intestines of HSIG-treated and control mic e when treatment was not started until 22 days of age. These results i ndicate that HSIG may be beneficial when given prophylactically; howev er, HSIG cannot eradicate cryptosporidia from mucosal surfaces in an e stablished infection.