THE EFFECT OF P-CHLOROANILINE ON LEUKOCYTES OF SHEEP PERIPHERAL-BLOODUNDER THE MIGRATION-INHIBITION TEST CONDITIONS

Toxic and immunotoxic effects of p-chloroaniline a metabolite of herbi cide monolinuron - were investigated in peripheral blood leucocytic su spensions of five sheep using a migration - inhibition test. The toxic effect of p-chloroaniline was recorded at concentrations 1.0 to 0.1 m g.ml(-1) and the immunotoxic one at concentrations 0.01 - 0.001 mg.1(- 1). The toxic effect was demonstrated by total inhibition of leucocyte migration. The immunotoxic effect, determined as mitogenic activation of leucocytes by phytohemagglutinine, concavaline A and lipopolysacch aride, was detected at 10 to 100-fold lower concentrations of p-chloro aniline than those which resulted in toxic effects.