THE S-LOCUS OF NICOTIANA-ALATA - GENOMIC ORGANIZATION AND SEQUENCE-ANALYSIS OF 2 S-RNASE ALLELES

Genomic clones encoding the S-2- and S-6-RNases of Nicotiana alata Lin k and Otto, which are the allelic stylar products of the self-incompat ibility (S) locus, were isolated and sequenced. Analysis of genomic DN A by pulsed-field gel electrophoresis and Southern blotting indicates the presence of only a single S-RNase gene in the N. alata genome. The sequences of the open-reading frames in the genomic and corresponding cDNA clones were identical. The organization of the genes was similar to that of other S-RNase genes from solanaceous plants. No sequence s imilarity was found between the DNA flanking the S-2- and S-6-RNase ge nes, despite extensive similarities between the coding regions. The DN A flanking the S-6-RNase gene contained sequences that were moderately abundant in the genome. These repeat sequences are also present in ot her members of the Nicotianae.