SEQUENCE-ANALYSIS OF 497 MOUSE-BRAIN ESTS EXPRESSED IN THE SUBSTANTIA-NIGRA

The use of subtracted, region-specific cDNA libraries combined with si ngle-pass cDNA sequencing allows the discovery of novel genes and faci litates molecular description of the tissue or region involved, We rep ort the sequence of 497 mouse expressed sequence tags (ESTs) from two subtracted libraries enriched for cDNAs expressed in the substantia ni gra, a brain region with important roles in movement control and Parki nson disease. Of these, 238 ESTs give no database matches and therefor e derive from novel genes, A further 115 ESTs show sequence similarity to ESTs from other organisms, which themselves do not yield any signi ficant database matches to genes of known function. Fifty-six ESTs sho w sequence similarity to previously identified genes whose mouse homol ogues have not been reported. The total number of ESTs reported that a re new for the mouse is 407, which, together with the 90 ESTs correspo nding to known mouse genes or cDNAs, contributes to the molecular desc ription of the substantia nigra. (C) 1997 Academic Press