THE BIOCHEMICAL CHARACTERISTICS OF BOAR SEMINAL PLASMA DURING HIGH EJACULATION FREQUENCY

The objective of this study was to determine the influence of two syst ems of semen-collection frequency in boars on the biochemical properti es of the seminal plasma. A total of eight monthly series of semen col lection were performed at different intensities in the two groups of b oars (Strzezek et al. 1995). Important changes were seen in the compos ition of the seminal plasma. These changes consisted of a lower conten t of total protein and Zn2+ ions in the seminal plasma. On the other h and, there was an increase in the pH value and changes were observed i n the secretion of acrosin inhibitors, anti-peroxidanting activity, as well as a decrease in acid and alkaline phosphatase activity. Electro phoretic analyses on molecular forms of alkaline phosphatase revealed a decreased activity of its epididymal form, slowly migrating towards the anode. The content of Zn2+ ions in seminal plasma showed statistic ally highly significant correlations with the content of total protein and various biochemical parameters of seminal plasma and spermatozoa. Not withstanding this, a high ejaculation frequency created new value s of correlation coefficients between the parameters discussed. Among them, the correlation coefficient existing between Zn2+ ion content in seminal plasma and intensity of H-3-AMD binding to chromatin of sperm atozoa disappeared. This would suggest a deconstruction in the mechani sm of binding and transport of Zn2+ ions following a high frequency of semen collection. The results indicate that high semen-collection fre quency disturbs important functions of the seminal plasma.