A. Chenn et Sk. Mcconnell, CLEAVAGE ORIENTATION AND THE ASYMMETRIC INHERITANCE OF NOTCH1 IMMUNOREACTIVITY IN MAMMALIAN NEUROGENESIS, Cell, 82(4), 1995, pp. 631-641
Neurons in the mammalian central nervous system are generated from pro
genitor cells near the lumen of the neural tube. Time-lapse microscopy
of dividing cells in slices of developing cerebral cortex reveals tha
t cleavage orientation predicts the fates of daughter cells. Vertical
cleavages produce behaviorally and morphologically identical daughters
that resemble precursor cells; these symmetric divisions may serve to
expand or maintain the progenitor pool. In contrast, horizontally div
iding cells produce basal daughters that behave like young migratory n
eurons and apical daughters that remain within the proliferative zone.
Notch1 immunoreactivity is distributed asymmetrically in mitotic cell
s, with Notch1 inherited selectively by the basal (neuronal) daughter
of horizontal divisions. These results provide cellular and molecular
evidence that cortical neurons are generated from asymmetric divisions
.