N. Inomata et al., EVOLUTIONARY RELATIONSHIPS AND SEQUENCE VARIATION OF ALPHA-AMYLASE VARIANTS ENCODED BY DUPLICATED GENES IN THE AMY LOCUS OF DROSOPHILA-MELANOGASTER, Genetics, 141(1), 1995, pp. 237-244
To infer the genealogical relationships of alpha-amylase electromorphs
of Drosophila melanogaster, we determined the nucleotide sequences of
a collection of electromorphs sampled throughout the world. On averag
e there were 1.0 amino acid substitutions between identical electromor
phs and 3.9 between different electromorphs, respectively. We found th
at the evolution of AMY(1) through AMY(6) electromorphs occurred by se
quential accumulation of single amino acid substitutions each causing
one charge difference. The nucleotide diversities at synonymous sites
within Amy(1),Amy(2),Amy(3),Amy(4) and Amy(6) were 0.0321, 0.0000, 0.0
355, 0.0059 and 0.0030, respectively. We also obtained evidence of gen
etic exchanges, such as intrachromosomal recombination, interchromosom
al recombination or gene conversion, between the mio duplicated Amy ge
nes as well as among the alleles.