Rb. Ray et al., HUMAN BREAST-CARCINOMA CELLS TRANSFECTED WITH THE GENE ENCODING A C-MYC PROMOTER-BINDING PROTEIN (MBP-1) INHIBITS TUMORS IN NUDE-MICE, Cancer research, 55(17), 1995, pp. 3747-3751
We have identified previously a gene from a human cervical carcinoma c
ell (HeLa) cDNA expression library that encodes a M(r) similar to 37,0
00 c-myc promoter-binding protein (MBP-1), which binds to the TATA box
sequences of c-myc P2 promoter and exerts a negative regulatory funct
ion by down-regulating c-myc expression. In normal human tissues, this
cloned gene showed variable expression, In this study, we have demons
trated that introduction of the MBP-1 gene into human breast carcinoma
cells reduced their ability to invade through a basement membrane mat
rix in vitro but did not alter their growth rate, Human breast carcino
ma transfected with MBP-1 cells showed a loss of anchorage-independent
growth and also suppressed tumor formation in athymic nude mice. Thes
e results suggest the possible involvement of MBP-1 as a tumor suppres
sor gene in human breast carcinoma cells.