WHOLE GENOME AMPLIFICATION OF SINGLE CELLS - MATHEMATICAL-ANALYSIS OFPEP AND TAGGED PCR

Authors
SUN FZ ARNHEIM N WATERMAN MS
Citation
Fz. Sun et al., WHOLE GENOME AMPLIFICATION OF SINGLE CELLS - MATHEMATICAL-ANALYSIS OFPEP AND TAGGED PCR, Nucleic acids research, 23(15), 1995, pp. 3034-3040
Citations number
8
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
23
Issue
15
Year of publication
1995
Pages
3034 - 3040
Database
ISI
SICI code
0305-1048(1995)23:15<3034:WGAOSC>2.0.ZU;2-O
Abstract
We construct a mathematical model for two whole genome amplification s trategies, primer extension preamplification (PEP) and tagged polymera se chain reaction (tagged PCR), An explicit formula for the expected t arget yield of PEP is obtained, The distribution of the target yield a nd the coverage properties of these two strategies are studied by simu lations. From our studies we find that polymerase with high processivi ty may increase the efficiency of PEP and tagged PCR.