GAS-PHASE INFRARED-SPECTRA OF 209-POLYCHLORINATED BIPHENYL CONGENERS USING GAS-CHROMATOGRAPHY WITH FOURIER-TRANSFORM INFRARED DETECTION - INTERNAL STANDARDIZATION WITH A C-13-LABELED CONGENER

The gas phase infrared spectra of biphenyl and 209 polychlorinated bip henyls (PCB) are observed; 30 are presented in graphical form so that their uniqueness can be appreciated. The spectra were observed with a gas chromatograph linked via a light pipe with a Fourier transform inf rared spectrometer. Unequivocal library matches (with match factors > 990 in 1000) can be performed with 50 ng of a congener injected into t he chromatograph. Quantitation with the technique was briefly investig ated with 2,2',5,5'-tetrachlorobiphenyl (BZ no. 52). Selective wavelen gth chromatograms of the most intense absorption (1457cm(-1)) were int egrated. Septuplicate injections showed that 20 ng of the compound cou ld be determined with +/-4% precision. The resolution of the spectrome ter was sufficient to distinguish between the C-13-labelled trichlorob iphenyl and the unlabelled material, indicating the possibility of int ernal or surrogate standardization of PCB analysis.