EFFECTS OF CISPLATIN AND SELENITE ON THE LEVEL OF THIOLS IN PIG-BLOODPLATELETS

The level of free sulfhydryl groups of glutathione and proteins in pig blood platelets after short lasting incubation (30 min.) of these cel ls with cisplatin (cis-diamminedichloroplatinum II) and sodium selenit e was investigated in vitro. The concentration of the platelet thiols after sodium selenite or cisplatin treatment distinctly decreased (p < 0.001). Pretreatment of blood platelets with sodium selenite at the l ow concentration (0.1 mu M) protected platelet glutathione against the reaction with cisplatin (p < 0.001). The present study shows that in blood platelets after the incubation with cisplatin the OS-platinum co mplex was formed. The results of our experiments demonstrated also tha t the extracellular GSH (1 mM) had the protective effect against cispl atin-induced peroxidation of platelet lipids.