The mobilization of N from reserve organs (leaves, roots, branches and
trunk) to developing new organs was studied at different moments of t
he growth cycle. Three-year-old Valencia Late orange trees (Citrus sin
ensis (L.) Osbeck) were grown individually outdoors in 150 L container
s filled with siliceous sand. Trees were irrigated with a nutrient sol
ution labelled with potassium nitrate with an enrichment of 4 atom N-1
5 % excess during a complete growth cycle. At the following year, plan
ts were irrigated with unlabelled nutrient solution, and harvested thr
oughout the growth cycle (flowering, fruit set, second flush, third fl
ush, and dormancy). Total N and N-15 analyses were carried out in the
different organs of the plants. The highest amounts of N were found in
leaves and roots (33-42% and 30- 38%, respectively). Distribution of
N-15 was similar to that obtained for total N (42 and 39% of total N-1
5 in leaves and roots, respectively) as is expected after a long perio
d of labelling. Old leaves were the main reserve organs, contributing
a 40-50% of the total N exported. Roots and aerial woody tissues expor
ted between 30-35% and 15-25% of total reserve N, respectively. N expo
rted by old leaves was approximately 57% of the N accumulated during t
he preceding year, while roots translocated 40% and trunk plus branche
s 35%. More than 70% of N accumulated in new organs during spring came
from N stored in old organs.