HPCZE OF NONIONIC COMPOUNDS USING A NOVEL ANIONIC SURFACTANT ADDITIVE

Excellent separations of nonionic organic compounds were obtained by a dding sodium dioctyl sulfosuccinate (DOSS) to an acetonitrile (similar to 40% v/v)-water electrolyte. Separation is based on differences in the strength of analyte-DOSS association ''complexes'' in solution, wh ich results in differences in effective electrophoretic mobility. Mice lle formation is not believed to occur. The effects of varying apparen t pH, applied voltage, acetonitrile concentration, and DOSS concentrat ion were studied with regard to electroosmotic mobility and effective electrophoretic mobility. Under optimum conditions, excellent separati ons of 23 organic compounds were obtained.