EFFECT OF THE INITIAL POTENTIAL RAMP ON THE ACCURACY OF ELECTROPHORETIC MOBILITIES IN CAPILLARY ELECTROPHORESIS

Authors
WILLIAMS BA VIGH G
Citation
Ba. Williams et G. Vigh, EFFECT OF THE INITIAL POTENTIAL RAMP ON THE ACCURACY OF ELECTROPHORETIC MOBILITIES IN CAPILLARY ELECTROPHORESIS, Analytical chemistry, 67(17), 1995, pp. 3079-3081
Citations number
17
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry → ACNP
ISSN journal
00032700
Volume
67
Issue
17
Year of publication
1995
Pages
3079 - 3081
Database
ISI
SICI code
0003-2700(1995)67:17<3079:EOTIPR>2.0.ZU;2-Z
Abstract
In several commercial capillary electrophoretic instruments, the poten tial is increased linearly at the beginning of a separation from zero to the programmed value. The ramp time can be as long as 20 s, resulti ng in grossly distorted mobility values when the migration times are c ommensurable with the ramp times. An expression is proposed to correct for the effects of the reduced held strength during the initial poten tial ramp.