THE GENOMIC IDENTIFICATION OF SOME MONOSOMICS OF AVENA-SATIVA L-CV SUN-II USING GENOMIC IN-SITU HYBRIDIZATION

Genomic in situ hybridization using total genomic DNA extracted from t he C genome diploid species Avena eriantha (2n = 2x = 14, genome CpCp) was used to identify monosomics (2n = 6x - 1 = 41) of the constituent genomes of the hexaploid cultivated oat A. sativa L. cv. Sun II (2n = 6x = 42, genomes AACCDD). The results demonstrate 3 AD/C and 6 C/AD c hromosome translocations, indicate that five of the missing monosomics are derived from the C genome, and show that there are duplicates wit hin the partial monosomic series. Chromosome polymorphisms between som e monosomic lines are also demonstrated.