Lm. Hyacinthe et al., INHIBITION OF BLADDER-TUMOR CELL IMPLANTATION IN CAUTERIZED UROTHELIUM, WITHOUT INHIBITION OF HEALING, BY A FIBRONECTIN-RELATED PEPTIDE (GRGDS), Annals of surgical oncology, 2(5), 1995, pp. 450-456
Background: Local recurrence after transurethral resection of bladder
tumors (TURB) is common and might be diminished if free tumor cells wi
thin the bladder are prevented from reattaching. Methods: In vitro inh
ibition of murine bladder tumor cells to an approximation of urothelia
l matrix with agents that might block attachment to components of the
extracellular matrix, and in vivo inhibition of attachment in cautery-
injured murine bladder. Results: GRGDS, (0.1-2.5 mg/ml), a fibronectin
-related peptide, mannose-6-phosphate, (0.1-20 mg/ml), a carbohydrate,
and heparin (1-625 units/ml) all inhibited attachment in vitro in a d
ose-dependent fashion. YIGSR (0.1-2 mg/ml), a laminin-related peptide,
did not. Mannose (10 mg/ml) did not significantly inhibit attachment
of tumor cells to cauterized urothelium in vivo, whereas there was a 7
7% reduction of attachment in bladders irrigated with GRGDS (6.25 mg/m
l) (p < 0.05), and the appearance of subsequent tumors in the bladder
was inhibited. Finally, GRGDS (6.25 mg/ml) did not inhibit healing of
the cautery ulcer.Conclusions: RGD-containing peptides may be useful a
s adjuvant therapy to decrease local recurrence after TURB and perhaps
in other circumstances in which tumor cells spilled into a wound or b
ody cavity threaten surgical success.