CHEMISTRY AND APPLICATIONS OF OLIGONUCLEOTIDE ANALOGS

This review is aimed at biochemists and molecular biologists, and cove rs the chemistry and key features involved in the solid-phase synthesi s of a variety of the better known DNA and RNA analogues by the phosph oramidite and H-phosphonate methods. A wide spectrum of biological app lications such as inhibition of gene expression, translation arrest, R NA processing, affinity purification of RNA-protein complexes, in situ hybridization, and synthetic ribozymes are then discussed in some det ail, enabling the molecular biologist to get an idea of what is possib le using the current technology.