PHYSICAL MAPPING OF THE 5S RIBOSOMAL-RNA MULTIGENE FAMILY IN 6X TRITICALE AND RYE - IDENTIFICATION OF A NEW RYE LOCUS

In situ hybridization was used to physically map the 5S rRNA multigene family in three selected lines of hexaploid triticale and five lines of diploid rye. Using this technique, evidence for a new locus on the 3RS arm of the three triticale lines was first obtained, as well as co nfirmation of the presence of 5S rRNA loci on wheat and rye chromosome s of homoeologous groups 1 and 5. The new locus on the 3RS arm was con firmed in two lines of rye, Secale cereale L., although it was not pre sent in the other rye varieties studied. We propose that the new 5S rR NA locus be referred to as 5SDna-R3.