SUBCELLULAR-LOCALIZATION OF PROTEIN-KINASE-C SUBSPECIES IN 0-2A LINEAGE GLIAL-CELLS IN CULTURE - COLOCALIZATION OF MARCKS PROTEIN AND PROTEIN-KINASE-C-DELTA TO CELL PROCESSES

0-2A lineage glial cells cultured from rat cerebral hemispheres have b een examined for the presence of eleven subspecies of the protein kina se C (PKC) family and their subcellular localisation studied by immuno fluorescence. With anti-C terminal peptide PKC subspecies-specific pol yclonal antibodies we confirmed the presence of the alpha, beta(II), d elta, epsilon and zeta subspecies and we also detected expression of t he iota and mu subspecies in this glial cell class. The alpha, beta(II ), epsilon, zeta, iota and mu subspecies were located in the glial cel l body in a diffuse pattern around the nucleus: PKC alpha was also loc ated within the nucleus. PKC delta was concentrated in fine processes extending from the cell where the PKC substrate and actin-crosslinking protein MARCKS (myristolated alanine-rich C kinase substrate) was als o detected. Double immunofluorescence labelling and confocal microscop y revealed that PKC delta and MARCKS protein were both concentrated in cell processes, especially at process tips, suggesting a possible fun ctional relationship.