R. Lopezalemany et al., BINDING OF PLASMINOGEN TO CORNEAL FIBROBLASTS AND THEIR EXTRACELLULAR-MATRIX - EVIDENCE FOR A RECEPTOR IN CELL-MEMBRANES, Fibrinolysis, 9(4), 1995, pp. 223-229
Several components of the fibrinolytic system play an important role i
n tissue remodelling after wounding of the cornea, Secretion of tissue
-type and urokinase-type plasminogen activators by corneal fibroblasts
has been described previously, In this work, we report the existence
of a plasminogen binding protein on the surface of cultured corneal fi
broblasts and in the extracellular matrix produced by these cells, Thi
s receptor was characterized on ligand-blots and localized by fluoresc
ence cytochemistry using biotinylated plasminogen and labelled strepta
vidin, Plasminogen binding to cells was specific, saturable, dose depe
ndent and inhibited by E-aminocaproic acid, indicating that the lysine
binding sites of plasminogen are involved in its interaction with the
cells and extracellular matrix, The plasminogen bound to corneal fibr
oblasts and their matrix was activated by t-PA secreted by the corneal
fibroblasts themselves, The receptor was characterized by ligand blot
ting using biotinylated plasminogen, It was insoluble in water solutio
n, but it could be solubilized in 1% SDS or 0.1% Triton X-100. The rec
eptor was purified by affinity chromatography on biotinylated plasmino
gen coupled to a streptavidin-agarose gel.