HYDROLYSIS OF MICROCRYSTALLINE CELLULOSE BY CELLULOLYTIC COMPLEX OF TRICHODERMA-REESEI IN LOW-MOISTURE MEDIA

Authors
SINEIRO J DOMINGUEZ H NUNEZ MJ LEMA JM
Citation
J. Sineiro et al., HYDROLYSIS OF MICROCRYSTALLINE CELLULOSE BY CELLULOLYTIC COMPLEX OF TRICHODERMA-REESEI IN LOW-MOISTURE MEDIA, Enzyme and microbial technology, 17(9), 1995, pp. 809-815
Citations number
22
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Enzyme and microbial technology → ACNP
ISSN journal
01410229
Volume
17
Issue
9
Year of publication
1995
Pages
809 - 815
Database
ISI
SICI code
0141-0229(1995)17:9<809:HOMCBC>2.0.ZU;2-F
Abstract
The activity of the extracellular cellulolytic complex of Trichoderma reesei upon crystalline cellulose was studied in the range of 4-10% mo isture (dry basis) and 20-90 degrees C temperature with the aid of a c entral composite experimental design. An empirical model was establish ed for both the endoglucanase and synergistic endo- and exoglucanase a ctivities with a significance level >95%. The thermal stability of the cellulolytic complex adsorbed onto cellulose at low moisture conditio ns was determined and compared with that of the enzyme in liquid form; the former was more stable.