DEVELOPMENT OF AN AUTOMATED FLOW-INJECTION CHEMILUMINESCENCE IMMUNOASSAY FOR HUMAN-IMMUNOGLOBULIN-G

The development of a competitive solid phase flow-injection immunoassa y with on-column chemiluminescence detection is described. The immunor eactor column consists of a transparent teflon tubing packed with immo bilized antibodies. It is placed in front of the photomultiplier tube. The entire assay takes place in this immunoreactor cell. The assay is performed by injection of a mixture of sample HIgG and acridinium lab eled HIgG followed by hydrogen peroxide to initiate the chemiluminesce nce reaction. One assay cycle including regeneration takes 7 min. The system is fully automated and controlled by computer to ensure exact t iming resulting in a precision of 1 to 3%, dependent on concentration. The detection limit was 7 fmol. The method was applied to serum sampl es without any sample clean-up. The average recovery from spiked serum samples was 103%.