SENSITIVE GAS CHROMATAGRAPHIC HIGH-RESOLUTION MASS-SPECTROMETRIC METHOD FOR THE DETERMINATION OF METHYLMALONIC ACID IN BOVINE PLASMA

Authors
YOUNG PB BLANCHFLOWER WJ HEWITT SA PRICE J KENNEDY DG
Citation
Pb. Young et al., SENSITIVE GAS CHROMATAGRAPHIC HIGH-RESOLUTION MASS-SPECTROMETRIC METHOD FOR THE DETERMINATION OF METHYLMALONIC ACID IN BOVINE PLASMA, Analyst, 120(8), 1995, pp. 2199-2201
Citations number
11
Categorie Soggetti
Chemistry Analytical
Journal title
Analyst → ACNP
ISSN journal
00032654
Volume
120
Issue
8
Year of publication
1995
Pages
2199 - 2201
Database
ISI
SICI code
0003-2654(1995)120:8<2199:SGCHMM>2.0.ZU;2-3
Abstract
A sensitive and highly specific method for the determination of methyl malonic acid (MMA) in bovine plasma is described. Following solvent ex traction and butylation, samples are analysed by gas chromatography an d detected using high-resolution mass spertrometry. The limit of detec tion for the assay was 0.025 mu mol l(-1) MMA and the recovery of adde d MMA ranged from 98 to 103%. The application of the method is demonst rated for the analysis of MMA in plasma taken from cattle that had bee n maintained on a cobalt-deficient diet for 64 weeks.