Cb. Baron et al., INOSITOL 1,4,5-TRISPHOSPHATE BINDING TO PORCINE TRACHEAL SMOOTH-MUSCLE ALDOLASE, The Journal of biological chemistry, 270(35), 1995, pp. 20459-20465
A cytoskeletal fraction of porcine tracheal smooth muscle (PTSM) was f
ound to contain > 90% of total cellular aldolase (fructose 1,6-bisphos
phate aldolase, EC 4.1.2.13) activity. PTSM aldolase was purified by D
EAE and inositol 1,4,5-trisphosphate (Ins(1,4,5)P-3) affinity chromato
graphy and found to react with an antibody directed against human aldo
lase C, but not anti aldolase A and B. The molecular mass of native al
dolase was about 138 kDa (on Sephacryl S-300); SDS-denatured enzyme wa
s 35 kDa (comigrated with rabbit skeletal muscle aldolase). Total cell
ular aldolase tetramer (aldolase(4)) content was 34.5 pmol/100 nmol li
pid P-i. Ins(1,4,5)P-3) binding activity coeluted with aldolase during
Sephacryl 300, DEAE, and Ins(1,4,5)P-3 affinity chromatography. Ins(1
,4,5)P-3 bound to purified aldolase (at 0 degrees C) in a dose-depende
nt manner over the range [Ins(1,4,5)P-3] 20 nM to 20 mu M, with maxima
l binding of 1 mol of Ins(1,4,5)P-3/mol aldolase, and a K-d of 12-14 m
u M. Fru(1,6)P-2 and Fru(2,6)P-2 displaced bound Ins(1,4,5)P-3) with a
50% inhibition at 30 and 170 mu M, respectively. Ins(1,3,4)P-3 (20 mu
M) and glyceraldehyde 3-phosphate (2 mM) were also potent inhibitors
of Ins(1,4,5)P-3 binding, but not inositol 4-phosphate or inositol 1,4
-bisphosphate (20 mu M each). Aldolase-bound Ins(1,4,5)P-3 may play a
role in phospholipase C-independent increases in free [Ins(1,4,5)P-3]