ROLE OF GLYCINE-1 AND LYSINE-2 IN THE GLYCATION OF BOVINE GAMMA-B-CRYSTALLIN - SITE-DIRECTED MUTAGENESIS OF LYSINE TO THREONINE

To determine the role of Gly-1 and Lys-8 of bovine gamma B crystallin in glycation and cross-linking, Lys-2 was changed to Thr by site-direc ted mutagenesis. A polymerase chain reaction was used to perform site directed mutagenesis on the third codon (AAG --> ACG) of bovine gamma B-crystallin cDNA. The wild type and mutant cDNAs were cloned into pMO N5743 and expressed in JM101 Escherichia coli cells, and the identity of gamma B-crystallin was confirmed by Western blotting after purifica tion by cation exchange high performance liquid chromatography. Glycat ion of gamma B-crystallin by [C-14]glucose was reduced significantly d ue to the mutation of Lys-2, supporting the view that Lys-2 is a major glycation site. Peptide mapping showed the presence of two major labe led peptides containing N-terminal sequences, and in the mutant these peptides had longer retention times and reduced radioactivity. Amino a cid analysis, after glycation with [C-14]glucose, revealed N-terminal glycine as the most predominant glycation site. Lys-8 was glycated slo wer than Gly-1 but faster than Lys-163. Glycation with DL-glyceraldehy de showed an important role for both Gly-1 and Lys-2 in the glycation- mediated gamma B-crystallin cross-linking.