CELL-MEDIATED REDUCTION AND INCOMPLETE MEMBRANE TRANSLOCATION OF DIPHTHERIA-TOXIN MUTANTS WITH INTERNAL DISULFIDES IN THE A-FRAGMENT

Active diphtheria toxin consists of two fragments, A and B, joined by a disulfide bond. The B fragment binds to cell surface receptors and a ids in the translocation of the enzymatically active A fragment to the cytosol. Normally, the toxin A fragment enters the cytosol from acidi c endosomes, but translocation can also be induced at the level of the plasma membrane by exposing cells with surface-bound toxin to low pH. Recently, we showed that disulfide bonds introduced into the A fragme nt by mutation are inhibitory for translocation. In the present work, we found that although the complete translocation of the A fragment is blocked, three mutant toxins underwent reduction of the interfragment disulfide bond upon low pH exposure, whereas the internal disulfide i n the A fragment remained intact. In the case of two of these mutants, the A fragment was released into the extracellular medium upon exposu re of cell-bound toxin to low pH. The pH profile for the release of th e mutant A fragments was the same as for translocation of wild-type A fragment to the cytosol, and the release was inhibited by conditions t hat interfere with A fragment translocation. In the case of the third mutant, which remained cell-associated upon reduction of the interfrag ment disulfide bond, a translocation intermediate was detected. The re sults show that the reduction of the interfragment disulfide bond can occur in the absence of complete translocation of the A fragment to th e cytosol, and they indicate that the reduction takes place at an earl y stage in the translocation process. Our findings suggest that the tr anslocation of the A fragment across the membrane is initiated at the C terminus.