IMMUNOENZYMATIC METHODS APPLIED TO THE SEARCH FOR BIOACTIVE TAXOIDS FROM TAXUS-BACCATA

Authors
GUO YW VANHAELENFASTRE R DIALLO B VANHAELEN M JAZIRI M HOMES J OTTINGER R
Citation
Yw. Guo et al., IMMUNOENZYMATIC METHODS APPLIED TO THE SEARCH FOR BIOACTIVE TAXOIDS FROM TAXUS-BACCATA, Journal of natural products, 58(7), 1995, pp. 1015-1023
Citations number
26
Categorie Soggetti
Chemistry,"Plant Sciences","Pharmacology & Pharmacy
Journal title
Journal of natural products → ACNP
ISSN journal
01633864
Volume
58
Issue
7
Year of publication
1995
Pages
1015 - 1023
Database
ISI
SICI code
0163-3864(1995)58:7<1015:IMATTS>2.0.ZU;2-4
Abstract
Polyclonal antibodies raised against 2'-succinyltaxol-bovine serum alb umin (BSA) conjugate were used for the immunodetection of bioactive ta xoids in chromatographic fractions of the stem bark extract of Taxus b accata. In addition to taxol, cephalomannine, and baccatin III, two ta xoids were isolated and their structures were elucidated as 4 alpha,7 beta-diacetoxy-2 alpha,9 alpha-dibenzoxy-5 beta,20-epoxy-10 beta,13 al pha,15-trihydroxy-11 (15-->1)-abeo-tax-11-ene[5] and taxol C [6] using spectroscopic methods.