NITRIC-OXIDE IS INVOLVED IN HEAT-INDUCED HSP70 ACCUMULATION

Heat shock potentiated the nitric oxide production (EPR assay) in the liver, kidney, heart, spleen, intestine, and brain. The heat shock-ind uced sharp transient increase in the rate of nitric oxide production p receded the accumulation of heat shock proteins (HSP70) (Western blot analysis) as measured in the heart and liver, In all organs the nitric oxide formation,vas completely blocked by the NO-synthase inhibitor N -omega-nitro-L-arginine (L-NNA), L-NNA also markedly attenuated the he at shock-induced accumulation of HSP70, The results suggests that nitr ic oxide is involved in the heat shock-induced activation of HSP70 syn thesis.