ANIONIC PHOSPHOLIPIDS CAN MEDIATE MEMBRANE INSERTION OF THE ANIONIC PART OF A BOUND PEPTIDE

Authors
LEENHOUTS JM VANDENWIJNGAARD PWJ DEKROON AIPM DEKRUIJFF B
Citation
Jm. Leenhouts et al., ANIONIC PHOSPHOLIPIDS CAN MEDIATE MEMBRANE INSERTION OF THE ANIONIC PART OF A BOUND PEPTIDE, FEBS letters, 370(3), 1995, pp. 189-192
Citations number
14
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
370
Issue
3
Year of publication
1995
Pages
189 - 192
Database
ISI
SICI code
0014-5793(1995)370:3<189:APCMMI>2.0.ZU;2-5
Abstract
The effect of anionic lipids on the membrane insertion of a carboxyl g roup on a specially designed palmitoylated peptide was studied, using tryptophan fluorescence. It is demonstrated that the negatively charge d membrane surface of mixed phosphatidylchoIine/phosphatidylglycerol s mall unilamellar vesicles enhances the protonation of the C-terminal c arboxyl group, and the subsequent insertion of that part of the peptid e.