SOLUBILITY DIAGRAM OF THE RHODOBACTER-SPHAEROIDES REACTION-CENTER AS A FUNCTION OF PEG CONCENTRATION

Authors
GAUCHER JF RIESKAUTT M REISSHUSSON F DUCRUIX A
Citation
Jf. Gaucher et al., SOLUBILITY DIAGRAM OF THE RHODOBACTER-SPHAEROIDES REACTION-CENTER AS A FUNCTION OF PEG CONCENTRATION, FEBS letters, 401(2-3), 1997, pp. 113-116
Citations number
35
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
401
Issue
2-3
Year of publication
1997
Pages
113 - 116
Database
ISI
SICI code
0014-5793(1997)401:2-3<113:SDOTRR>2.0.ZU;2-3
Abstract
In order to quantify the effect of polyethylene glycol 4000 (PEG) on t he solubility of an integral membrane protein, we have crystallized th e photochemical reaction center from Rhodobacter sphaeroides Y by batc h method on a large range of PEG, The measurement of the solubility di agram display a semi-logarithmic dependence of solubility versus PEG c oncentration, Comparison of our results with previously published ones [Odahara, T., Ataka, M. and Katsura, M. (1991) Acta Cryst. D50, 639-6 42] suggests a notable effect of additional 1,2,3-heptane-triol and/or temperature on photochemical reaction center solubility.