CHARACTERIZATION OF A NEW CELL-BOUND ALPHA-AMYLASE IN BACILLUS-SUBTILIS 168-MARBURG THAT IS ONLY IMMUNOLOGICALLY RELATED TO THE EXOCELLULARALPHA-AMYLASE

Authors
HADDAOUI E PETITGLATRON MF CHAMBERT R
Citation
E. Haddaoui et al., CHARACTERIZATION OF A NEW CELL-BOUND ALPHA-AMYLASE IN BACILLUS-SUBTILIS 168-MARBURG THAT IS ONLY IMMUNOLOGICALLY RELATED TO THE EXOCELLULARALPHA-AMYLASE, Journal of bacteriology, 177(17), 1995, pp. 5148-5150
Citations number
9
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
177
Issue
17
Year of publication
1995
Pages
5148 - 5150
Database
ISI
SICI code
0021-9193(1995)177:17<5148:COANCA>2.0.ZU;2-P
Abstract
Immunoblot analysis of Bacillus subtilis cell extracts with polyclonal antibodies, raised against purified exocellular alpha-amylase, reveal ed one protein species of 82,000 Da. This protein was found even in ce lls in which the amyE gene, encoding exocellular alpha-amylase, was di srupted. Isolated from the membrane fraction, the 82,000-M(r) protein displayed an alpha-amylase activity in vitro.