Tj. Zupancic et al., ISOLATION OF PROMOTERS FROM BREVIBACTERIUM-FLAVUM STRAIN MJ233C AND COMPARISON OF THEIR GENE-EXPRESSION LEVELS IN B-FLAVUM AND ESCHERICHIA-COLI, FEMS microbiology letters, 131(2), 1995, pp. 121-126
A promoter probe shuttle vector suitable for the isolation of promoter
elements from coryneform bacteria was constructed. This vector carrie
d the neomycin phosphotransferase (NPTII) gene from transposon Tn5 as
a reporter gene, and was capable of replication in both Escherichia co
li and Brevibacterium flavum. The vector was used in the construction
of a B. flavum library of 899 independently isolated promoter clones.
Promoters with a wide range of activities in B. flavum, including some
very strong promoter elements, were isolated. Comparative analysis su
ggests that significant differences between B. flavum and E. coli may
exist in the determinants of promoter strength.