Transformation of soybean [Glycine max (L.) Merr.] with a gene encodin
g a glyphosate-tolerance 5-enolpyruvylshikimate-3-phosphate synthase e
nzyme from Agrobacterium sp. strain CP4 resulted in the development of
glyphosate-tolerant line 40-3-2. Glyphosate (N-phosphonomethyl glycin
e) is the active ingredient of Roundup herbicide. Line 40-3-2 was yiel
d tested at 17 locations in 1992, 23 locations in 1993, and 18 locatio
ns in 1994. At those locations, broadcast applications of glyphosate a
t various rates were made over 40-3-2 or its derivatives from early ve
getative growth to pod fill. No significant yield reduction was observ
ed as a result of the glyphosate treatment at any of the locations. De
velopment of glyphosate-tolerant soybean promises to provide the farme
r with access to a new weed control system that should result in lower
production costs and reliable weed control under a wide range of cond
itions.