CONFOCAL SPECTRAL IMAGING ANALYSIS OF THE DISTRIBUTION OF MITOXANTRONE WITHIN THE SINGLE LIVING CELL AS A FUNCTION OF CELL-CYCLE

Authors
FEOFANOV A FLEURY F SHARONOV S MORJANI H NABIEV I MANFAIT M
Citation
A. Feofanov et al., CONFOCAL SPECTRAL IMAGING ANALYSIS OF THE DISTRIBUTION OF MITOXANTRONE WITHIN THE SINGLE LIVING CELL AS A FUNCTION OF CELL-CYCLE, Journal of trace and microprobe techniques, 13(3), 1995, pp. 241-243
Citations number
4
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of trace and microprobe techniques → ACNP
ISSN journal
07334680
Volume
13
Issue
3
Year of publication
1995
Pages
241 - 243
Database
ISI
SICI code
0733-4680(1995)13:3<241:CSIAOT>2.0.ZU;2-T
Abstract
Confocal fluorescence spectral imaging technique was used to analyse t he intracellular molecular interactions and distribution of mitoxantro ne (MTX) within synchronised K562 cancer cells in the S-, G(2)- and M- phases of cell cycle. The spectral decomposition of the original fluor escence signal coming from a microvolume has been carried out to calcu late the intracellular distribution of free MTX and MTX/DNA complex. T he analysis of the distribution of MTX in different phases of the cycl e has been applied to study the phase-dependent changes of intracellul ar molecular interaction of MTX.