EFFECTS OF BUFFER CONCENTRATION ON THE ELECTROPHORETIC BEHAVIORS OF SMALL PEPTIDES IN CAPILLARY ZONE ELECTROPHORESIS

It was found that unlike the electroosmotic flow, the electrophoretic mobilities of small peptides in capillary zone electrophoresis (CZE) w ere invariant over the buffer concentration range studied. Migration o f these peptides in CZE is a electroosmotic-governed process. The quan titative linear relationships between the logarithm of migration times (log t(m)) and the reciprocal of column temperature were investigated under different buffer concentrations to study the activation energie s of diffusion (AED) as the function of the buffer concentration. The preexponential factors were quantitatively correlated with the structu ral parameters of the peptides studied. (C) 1995 John Wiley & Sons, In c.