MICROPREPARATIVE FOCUSING OF PROTEINS IN CARRIER-AMPHOLYTE-FREE SOLUTION WITH ELECTRICALLY CONTROLLED COMPOSITIONS OF ELECTROLYTES

Authors
POSPICHAL J CHMELIK J DEML M
Citation
J. Pospichal et al., MICROPREPARATIVE FOCUSING OF PROTEINS IN CARRIER-AMPHOLYTE-FREE SOLUTION WITH ELECTRICALLY CONTROLLED COMPOSITIONS OF ELECTROLYTES, The Journal of microcolumn separations, 7(3), 1995, pp. 213-219
Citations number
19
Categorie Soggetti
Chemistry Analytical
Journal title
The Journal of microcolumn separations → ACNP
ISSN journal
10407685
Volume
7
Issue
3
Year of publication
1995
Pages
213 - 219
Database
ISI
SICI code
1040-7685(1995)7:3<213:MFOPIC>2.0.ZU;2-I
Abstract
Three proteins (myoglobin, ribonuclease, and cytochrome C) were concen trated, separated, and isolated from a dilute mixture by electrically controlled focusing in carrier-ampholyte-free solution. Electrolytes w ere modified at both sides of the capillary. The described method is s uitable for the micropreparation of hundred-mu g quantities of protein s in times comparable to other capillary techniques (tens of minutes). (C) 1995 John Wiley & Sons, Inc.