COMPARISON OF SPHERICALLY AND IRREGULARLY SHAPED STATIONARY-PHASE PACKINGS IN MICROCOLUMN LIQUID-CHROMATOGRAPHY

Spherically and irregularly shaped reversed phase packings were used t o slurry pack capillary fused silica columns. The selection of the pac king solvents was based on the colloidal properties of the stationary phase particles and investigated by sedimentation experiments. The chr omatographic performance of the microcolumns was measured with convent ional parameters from plate and rate theories, and the column resistan ce parameter and separation impedance. Also studied was the time of an alysis. The performance of spherical and irregular packings was compar able with a light preference for spherically shaped materials when tim e of analysis is concerned. (C) 1995 John Wiley & Sons, Inc.