ANALYSIS OF ESTERASE ZYMOGRAMS OF FUSARIUM-SAMBUCINUM AND RELATED SPECIES

Esterase zymograms were obtained following polyacrylamide slab gel ele ctrophoresis of protein extracts Fusarium sambucinum and related speci es originating from different geographic locations and different matri ces. The sites of esterase activity were recorded, and the R(f)s were calculated. The data were used for the construction of phenograms by c luster analysis and nonlinear mapping by computerized classification t echniques. The fifteen isolates of F. sambucinum, the eight isolates o f F. torulosum and the six isolates of F. spec. nov. each had identica l profiles, and are therefore electrophoretically distinct species. Th e isolates of F. sarcochroum, one of F. sambucinum sensu late (BBA 642 80) and fifteen isolates of F. sambucinum were electrophoretically ind istinguishable from each other. We assume they are synonymous. The iso late of F. bactridioides, one of F. sambucinum sensu late (BBA 64993) and eight isolates of F. torulosum had uniform EST patterns, therefore the two species are electrophoretically identical. We assume they are also synonymous. The remaining three isolates of F. sambucinum sensu late are somewhat closely related to F, sambucinum isolates on the bas is of our investigations.