GENETIC-FACTORS INFLUENCING THE REGENERATION ABILITY OF RYE (SECALE-CEREALE L) .2. IMMATURE EMBRYOS

Immature embryos of seven rye inbred lines were cultured on modified M S medium containing 3 mg/dm(-3) 2,4-D. According to the in vitro respo nse lines were divided into four groups: (1) those producing non-embry ogenic callus (NEC) from above 30% of the embryos and having a high ra te of non-responding (NR) embryos, (2) those producing non-embryogenic callus (NEC) from the majority of embryos, (3) those producing NEC by the majority of embryos with a high percentage of calli regenerating roots, (4) those producing embryogenic callus (EC) and regenerating pl ants by above 50% of the embryos. The inheritance of these response ty pes was analysed in F-1, F-2, and F-3 generations of crosses of some l ines. The results obtained indicate that EC production and both plant and root regeneration are determined by recessive genes whereas the re duced ability for NEC production most probably by dominant genes. The lack of response is controlled by at least two interacting genes.