PROTECTIVE EFFECT OF HEAT-SHOCK PRETREATMENT WITH HEAT-SHOCK PROTEIN INDUCTION BEFORE HEPATIC WARM ISCHEMIC-INJURY CAUSED BY PRINGLES MANEUVER

Background. Induction of heat shock proteins is thought to have a>cyto prorective effect against environmental stress and to result in a bett er ischemic tolerance. The protective ability of heat exposure and hea t shock protein 72 (HSP 72) induction before warm ischemia caused by P ringle's maneuver was evaluated in rats. Methods. Heat exposed rats (H S) were compared with control animals (C). The gene expression (messen ger RNA) of HSP 72 and HSP 72 were detected by Northern and Western bl ot analyses. During 40 minutes of in situ reperfusion, liver energy me tabolism and levels of standard liver enzymes were evaluated. The surv ival rate was determined after postoperative day 7. Results. After hea t exposure and recovery, messenger RNA of HSP 72 and HSP 72 can be det ected strongly in HS group but not in C group. During reperfusion HS g roup exhibited a significantly (p < 0.01) improved energy metabolism, and the release of liver enzymes was significantly (p < 0.001) reduced compared with C group. Seven-day survival rare was 100% in HS group b ut at 50% was significantly impaired (p < 0.05) in C group. Conclusion s. Heat exposure associated with HSP induction has a significant prote ctive effect against warm ischemic liver injury, which results in a re levant improvement of survival rate.