H. Chaabihi et al., A NEW VECTOR FOR EFFICIENT GENERATION OF P10-SINGLE-LATE-PROMOTER RECOMBINANT BACULOVIRUSES, Journal of virological methods, 63(1-2), 1997, pp. 1-7
Citations number
16
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
A new baculovirus (BacTen) was constructed in order to generate p10 re
combinant expression vectors al high frequency. This virus is an AcMNP
V derivative, with the polyhedrin gene deleted and thus exhibiting p10
promoter as a single strong late promoter. The polyhedrin coding sequ
ence was re-inserted subsequently under the control of the p10 promote
r, in place of the p10 coding sequence. Two flanking Bsu36I restrictio
n sites were inserted together with the polyhedrin coding region. BacT
en can, therefore, be efficiently restricted at the p10 locus and used
in co-transfection experiments along with p10 transfer vectors carryi
ng the foreign gene to be expressed. It is shown with three independen
t transfer vectors, that the proportion of recombinants in the viral p
rogeny can be as high as 80%. The BacTen baculovirus represents a new
powerful tool for the generation of p10 promoter based expression vect
ors, in a system without the background of considerable production of
very late proteins. Copyright (C) 1997 Elsevier Science B.V.