Sf. Osman et al., METHOD FOR THE PRODUCTION AND CHARACTERIZATION OF TOMATO CUTIN OLIGOMERS, Journal of agricultural and food chemistry, 43(8), 1995, pp. 2134-2137
A series of oligomeric fragments can be obtained by partial alkaline h
ydrolysis of cutin. The oligomers obtained from tomato cutin were anal
yzed by high-performance liquid chromatography-mass spectrometry and t
he major products were characterized as linear dimeric esters of omega
,8-, omega,9-, and omega,10-dihydroxyhexadecanoic acids, the major mon
omers of tomato cutin. Analysis of the products obtained from tomato c
utin by treatment with cutinase suggests an exo mode of depolymerizati
on for this enzyme.