DEVELOPMENT OF MONOCLONAL-ANTIBODIES FOR RED SNAPPER (LUTJANUS-CAMPECHANUS) IDENTIFICATION USING ENZYME-LINKED-IMMUNOSORBENT-ASSAY

Two murine IgG1 monoclonal antibodies (McAbs), C1C1 and C2A2, against red snapper protein a were produced using hybridoma technique. McAb C1 C1 showed strong reactivity with red snapper, vermilion snapper (VS), lane snapper (LS), mutton snapper (MS), and yellowtail snapper (YS), a nd it could be used in ELISA and immunodot blotting to screen these fi sh species from 36 commonly consumed seafood and meat samples. Since C 2A2 did not react as strongly with VS, LS, MS, and YS as with red snap per, it could be used to separate red snapper from the remaining four species. The combined use of these two McAbs in a blind study using EL ISA correctly identified red snapper from 24 various seafood and meat extracts. Furthermore, the identities of 24 commercial fish samples cl aimed to be red snapper, MS, Caribbean red snapper, and other various red snappers by seafood retailers were verified using ELISA with these two McAbs and thin-layer and slab IEF gel electrophoresis.