3-DIMENSIONAL STRUCTURE OF THE SINGLE-STRANDED DNA-BINDING PROTEIN ENCODED BY GENE-V OF THE FILAMENTOUS BACTERIOPHAGE-M13 AND A MODEL OF ITS COMPLEX WITH SINGLE-STRANDED-DNA
Rnh. Konings et al., 3-DIMENSIONAL STRUCTURE OF THE SINGLE-STRANDED DNA-BINDING PROTEIN ENCODED BY GENE-V OF THE FILAMENTOUS BACTERIOPHAGE-M13 AND A MODEL OF ITS COMPLEX WITH SINGLE-STRANDED-DNA, FEMS microbiology reviews, 17(1-2), 1995, pp. 57-72
Gene V protein (GVP) of the filamentous bacteriophage M13 is a single-
stranded DNA (ssDNA) binding protein that both regulates virus DNA rep
lication and gene expression and that, upon cooperative binding to the
viral genome, forms a regular left-handed superhelical polymer in whi
ch the GVP dimers are arrayed at the outside and the ssDNA strands on
the inside. It is 87 amino acids long and occurs in solution as a homo
dimer. The solution structure of the homodimer of the GVP mutant Tyr41
-His has recently been elucidated by nuclear magnetic resonance and X-
ray crystallographic techniques (Folkers et al., (1994) J. Mol. Biol.
236, 229-246; Skinner, M.M. et al. (1994) Proc. Natl. Acad. Sci. USA 9
1, 2071-2075). The monomer consists of a distorted five-stranded P-bar
rel from which three major loops protrude; one of these is involved in
dimerization, another in DNA binding and a third in cooperative prote
in-protein interactions. To derive a model for the complex between vir
al DNA and GVP, a contact analysis and a series of restrained molecula
r dynamics simulations were employed. Contact analysis served to deter
mine the helix parameters that permit the energetically most favourabl
e packing of the protein molecules. Subsequently, the superhelix was b
uilt into which two extended DNA strands were modelled using restraine
d molecular dynamics. Specific constraints, based on nuclear magnetic
resonance spin label experiments, were included to ensure that the DNA
would position itself into the binding groove of the protein. The lef
t-handed model presented is highly consistent with existing biophysica
l and biochemical data. A description of the protein-protein interface
is given and the interaction between the protein and DNA is discussed
in view of the derived model. Tn addition, it is described that, on t
he basis of the available experimental data and not imposing the left-
handedness of the nucleoprotein complex, it is feasible also to build
a plausible model for the complex which exhibits the opposite, i.e. ri
ght-handed, helical sense. This right-handed structure features charac
teristics highly similar to those of the left-handed complex. The mean
ing of the helical models regarding the biological role GVP fulfils in
the phage replication process is discussed.