Sc. Chaiyaroj et al., PRODUCTION OF MONOCLONAL-ANTIBODY SPECIFIC TO CAMPYLOBACTER-JEJUNI AND ITS POTENTIAL IN DIAGNOSIS OF CAMPYLOBACTER ENTERITIS, Asian Pacific Journal of Allergy and Immunology, 13(1), 1995, pp. 55-61
A monoclonal antibody (MAb 3G6) specific for Campylobacter jejuni and
Campylobacter coli was produced by immunizing BALB/c mice with a local
strain of C. jejuni (28.1), No cross-reactivity was observed with Ent
erobacteriaceae controls, By immunoprecipitation, MAb 3G6 identified a
major protein band of molecular weight 45 kDa and also gave a slight
reactivity with 30 and 55 kDa proteins. Using an indirect enzyme-linke
d immunosorbent assay, MAb 3G6 was able to detect C. jejuni suspended
in stool without cross reactivity to 14 other enteropathogenic bacteri
a suspended, normal flora in fecal suspension, or fecal debris, In the
analysis of fifty clinical specimens, MAb 3G6 detected most positive
samples with the exception of one which possessed very low Campylobact
er concentration and gave no reactivity to negative samples, demonstra
ting its high specificity to C. jejuni and C. coli. MAb 3G6 may be sui
table as a new tool for the development of diagnostic method for Campy
lobacter infection.