THE CORRELATION OF THYMIDINE PHOSPHORYLASE-ACTIVITY WITH THE EXPRESSION OF INTERLEUKIN-1-ALPHA, INTERFERON-ALPHA AND INTERFERON-GAMMA IN HUMAN COLORECTAL-CARCINOMA
Y. Takebayashi et al., THE CORRELATION OF THYMIDINE PHOSPHORYLASE-ACTIVITY WITH THE EXPRESSION OF INTERLEUKIN-1-ALPHA, INTERFERON-ALPHA AND INTERFERON-GAMMA IN HUMAN COLORECTAL-CARCINOMA, Cancer letters, 95(1-2), 1995, pp. 57-62
Thymidine phosphorylase (dThdPase) is an angiogenic enzyme and seems t
o be related to an angiogenesis in human colorectal carcinoma, The inc
idence of dThdPase-positive cells was significantly correlated with mi
crovessel count in 21 human colorectal carcinomas, Interleukin 1 alpha
(IL-1 alpha), tumor necrosis factor alpha (TNF-alpha), interferon alp
ha (IFN-alpha) interferon gamma (IFN-gamma) induce dThdPase activity i
n human cancer cell lines, To study whether this phenomenon occurs in
the human colorectal carcinomas, we examined the correlation between d
ThdPase activity and the expression levels of IL-1 alpha, TNF-alpha, I
FN-alpha and IFN-gamma in colorectal carcinoma tissues, dThdPase activ
ity was assayed by the methods of Friedkin and Robert, and the express
ion level of IL-1 alpha, TNF-alpha, IFN-alpha and IFN-gamma was determ
ined by ELISA. dThdPase activity was significantly correlated with the
amount of IL-1 alpha (n = 19, r = 0.347, P = 0.0001), INF-alpha (n =
18, r = 0.717, P = 0.0008), and IFN-gamma (n = 4, r = 0.9777, P = 0.02
34) in human colorectal carcinomas, However, the dThdPase activity was
not correlated with the amount of TNF-a (n = 21, r = 0.235, P = 0.268
2), These results suggested that the expression levels of IL-1 alpha,
IFN-alpha and IFN-gamma are correlated with dThdPase activity in human
colorectal carcinomas and that these cytokines may cause angiogenesis
by inducing the expression of dThdPase.